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RMRC No. RMRC13387 Order
Strain/Stock Designation B6.129-Pkd1tm1Sji/Narl
Status
SP| (Cryopreserved or freeze-dried sperm)
Availability Level4
Pricing Industry：14,710NTD　Academia: 14,710NTD　NSTC: 10,300NTD
Last Update Date 2024/3/28
Analysis Data
  • Description
  • Genes &
    alleles
  • Phenotype
  • Health &
    husbandry
  • References
  • Specific Term
    and Conditions
  • Construct map
Species ： laboratory mouse
Strain Type ： COI (coisogenic strain),MSR (mutant strain)
Mutant Type ： TM (Target Mutation)
Other Names ： Pkd1 L3
Background Strain ： C57BL/6
Strain Description ：
1.  This mouse model is suitable for further studies of progression and therapeutic interventions of autosomal-dominant polycystic kidney disease (ADPKD).
2.  Targeting vector was designed to introduce a single loxP site and a loxP-flanked mc1-neomycine cassette into intron 30 and intron 34 of Pkd1 gene respectively. The targeting vector was electroporated into E14 embryonic stem (ES) cells derived from 129/Ola strain. The mice then backcrossed to C57BL/6J strain over ten generations.
3.  Mice homozygous for mutant alleles expressing a low level of polycystin-1 appeared normal at birth, but they exhibited distended abdomens and short stature at weaning. Kidney enlargement and massive renal cysts were observed as early as postnatal day14. Homozygotes died within postnatal 21 days.But this renal cystic disease was not found in heterozygotes.
Depositor ：
Si-Tse Jiang / 蔣思澈
Email ： stjiang@narlabs.org.tw
Allele Symbol ： Pkd1tm1Sji
Allele Name ： polycystin 1, transient receptor potential channel interacting, target mutation 1, National Applied Research Laboratories
Allele Description ：
Strain of Origin ： 129
Gene Symbol ： Pkd1
Gene Name ： polycystin 1, transient receptor potential channel interacting
Chromosome ： 17
Genotyping Method ：            
Genotyping NLAC ：            
Research Application ：
Internal/ Organ Cell and Developmental Biology
Construction Technologies ：
Cre-lox system
Animal Health Reports ：
Colony Maintenance
Breeding & Husbandry 		：
Colony Maintenance
Mating System 		： hetero/hemi x wild type
References ： PubMed ID: 16400024

Specific Terms of Use ：
有特別規定
本生物材料僅限使用於學術研究。寄存者對於分讓申請人使用本材料 所獲得之研究成果，不主張任何專利、智慧財產權及其他財產權
DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of BIOLOGICAL RESOURCE for academic research purpose.
分讓申請人公開發表使用本材料所獲得之論文或其他研究成果時，應引用下述已發表之論文： Am J Pathol . 2006 Jan;168(1):205-20 Defining a link with autosomal-dominant polycystic kidney disease in mice with congenitally low expression of Pk
Should the use of this material result in one or more scientific
publication(s) the recipient agrees to cite the paper(s)： Am J Pathol . 2006 Jan;168(1):205-20 Defining a link with autosomal-dominant polycystic kidney disease in mice with congenitally low expression of Pkd1
Construct Map ：
 
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