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RMRC No.

RMRC13258

Order

Strain/Stock Designation

NOD-Tg(INS-anti-Ctla4scFv)Sytwu

Status

EM| (Cryopreserved Embryos)

Availability

Level4

Pricing

(Embryos) 　International：560USD
(Cryo Recovery) 　International：760USD

Last Update Date

2015/9/17

Analysis Data

Description
Genes &
alleles
Phenotype
Health &
husbandry
References
Specific Term
and Conditions
Construct map

Species

：

laboratory mouse

Strain Type

：

COI (coisogenic strain),MSR (mutant strain)

Mutant Type

：

TG (Transgenic)

Other Names

：

Background Strain

：

NOD/Sytwu

Strain Description

：

1. Transgene consists of human insulin promoter, the first and part of the second noncoding exons of insulin, an intron from rabbit betaglobin, membrane-bound agonistic anti-CTLA-4 scFv/eB7/B7TM, and a poly-A signal from rabbit betaglobin.
2. Anti-CTLA-4 scFv is from 4F10 hybridoma with two mutations in the L chain variable region, a myc epitope, an extracellular portion, a transmembrane (TM) domain and the cytosolictail (CT) of murine B7-1.
3. Anti-Clta4scFv only detected in pancreatic beta cells with RT-PCR.
4. Carried 5-10 copies of the chimeric transgene.
5. Delay in onset and inhibits the development of autoimmune diabetes in NOD mice.

Depositor

：

Huey-Kang Sytwu

司徒惠康

：

sytwu@ndmctsgh.edu.tw

Allele Symbol	：	Tg(INS-anti-Ctla4scFv)Sytwu

Allele Name	：	Transgenic insertion, Huey-Kang Sytwu

Allele Description	：






Strain of Origin	：	NOD/Sytwu

Gene Symbol	：	Ctla4

Gene Name	：	cytotoxic T-lymphocyte-associated protein 4

Chromosome	：	UN






Genotyping Method	：	Download
Genotyping NLAC	：

Research Application

：

Cancer	Immunology and Inflammation
Diabetes and Obesity	Endocrine

Construction Technologies

：

Animal Health Reports	：

Colony Maintenance Breeding & Husbandry	：

Colony Maintenance Mating System	：	hetero/hemi x wild type

References

：

PubMed ID: 19635924

Specific Terms of Use

：

有特別規定
本生物材料僅限使用於學術研究。寄存者對於分讓申請人使用本材料所獲得之研究成果，不主張任何專利、智慧財產權及其他財產權
DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of BIOLOGICAL RESOURCE for academic research purpose.

分讓申請人公開發表使用本材料所獲得之論文或其他研究成果時，應引用下述已發表之論文： Transgenic Expression of Single-Chain Anti-CTLA-4 Fv on β Cells Protects Nonobese Diabetic Mice from Autoimmune Diabetes

Should the use of this material result in one or more scientific
publication(s) the recipient agrees to cite the paper(s)： Transgenic Expression of Single-Chain Anti-CTLA-4 Fv on β Cells Protects Nonobese Diabetic Mice from Autoimmune Diabetes

分讓申請人公開發表使用本材料所獲得之論文或其他研究成果時，應揭露並致謝本材料係由Dr. Huey-Kang Sytwu所提供

Should the use of this material result in one or more scientific publication(s) the Recipient Investigator agrees to mention and acknowledge Dr. Huey-Kang Sytwu

分讓申請人向國研院動物中心國家實驗鼠種原庫(NLAC-RMRC)申請本材料時，應附上寄存人簽名之授權分讓同意書作為附件；NLAC- RMRC 在取得寄存人書面同意後，始進行此材料之分讓。
The recipient should obtain a written consent from the depositor of the BIOLOGICAL RESOURCE, prior to NLAC-RMRC accepting the material distribution request.
Depositor Email: sytwu@ndmctsgh.edu.tw

Construct Map

：