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RMRC No. RMRC13432 Order
Strain/Stock Designation CD1(ICR)-Akr1A1Tg(CAG-EGFP)Meltw/Narl
Status
EM| (Cryopreserved Embryos)
Availability Level4
Pricing (Embryos) 　International：560USD
(Cryo Recovery) 　International：760USD
Last Update Date 2026/10/29
Analysis Data
  • Description
  • Genes &
    alleles
  • Phenotype
  • Health &
    husbandry
  • References
  • Specific Term
    and Conditions
  • Construct map
Species ： laboratory mouse
Strain Type ： MSK (mutant stock),CON (congenic strain)
Mutant Type ： TM (Target Mutation)
Other Names ： Akr1A1 KO、Akr1A1eGFP/eGFP
Background Strain ： CD-1 or ICR mice
Strain Description ：
1.  The original founder of the outbred CD1 strain was generated via pronuclear microinjection with a 3,253 bp pCAG-eGFP transgenic construct, leading to strong and ubiquitous green fluorescence expression across multiple organs, including the brain, heart, lungs, Deposit Application Form－NCB-TS-4-001-D-05 kidneys, spleen, and gonads. The offspring maintained in CD1(ICR) background.
2.  One of the key features of this strain is the sexually dimorphic expression of EGFP in the liver. Post-puberty, male mice exhibit higher EGFP expression, with fluorescent hepatocytes extending to the boundaries of the hepatic lobule. In contrast, in female mice, EGFP expression is primarily restricted to hepatocytes surrounding the central vein of the lobule.
3.  The transgene was inserted at the D1 band of mouse chromosome 4, causing a ~30 kb deletion at this locus and disrupting Akr1a1 gene expression. In homozygous transgenic mice, the loss of Akr1a1, a key enzyme in the ascorbic acid (vitamin C) biosynthesis pathway, results in significant serum vitamin C deficiency.
4.  The vitamin C deficiency caused by Akr1a1 disruption, leads to a notable osteoporotic phenotype, even at a young age (12 weeks). Affected mice exhibit severe trabecular bone loss, reduced bone mineral density (BMD), decreased cortical bone thickness, and enlargement of the bone marrow cavity in the distal femur. Additionally, the proximal femur, including the femoral head and neck, shows substantial bone mass loss. These abnormalities are associated with impaired bone formation and decreased serum osteocalcin levels.
5.  In summary, the sexually dimorphic EGFP expression in this transgenic strain offers a model for investigating sex-specific gene regulation, particularly in liver biology and disease. Meanwhile, the ascorbic acid deficiency and resulting osteoporosis caused by Akr1a1 disruption may provide insights into bone formation and resorption mechanisms, with potential to therapeutic developments.
Depositor ：
Chuan-Mu Chen / 陳全木
Email ： chchen1@dragon.nchu.edu.tw
Allele Symbol ： Akr1A1Tg(CAG-EGFP)Meltw
Allele Name ： aldo-keto reductase family 1, member A1,transgenic insertion, CAG-EGFP,Chuan-Mu Chen
Allele Description ：
Strain of Origin ： CD-1 mice
Gene Symbol ： Akr1A1
Gene Name ： aldo-keto reductase family 1, member A1
Chromosome ： 4
Genotyping Method ： Download            
Genotyping NLAC ：            
Research Application ：
Cardiovascular Metabolism
Genetics Research
Construction Technologies ：
Animal Health Reports ：
Colony Maintenance
Breeding & Husbandry 		： No
Colony Maintenance
Mating System 		： homo x hetero/hemi
References ： PubMed ID: 27087367
PubMed ID: 28060768
PubMed ID: 36252352
Specific Terms of Use ：
有特別規定
本生物材料僅限使用於學術研究。寄存者對於分讓申請人使用本材料 所獲得之研究成果，不主張任何專利、智慧財產權及其他財產權
DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of BIOLOGICAL RESOURCE for academic research purpose.
分讓申請人公開發表使用本材料所獲得之論文或其他研究成果時，應引用下述已發表之論文： PubMed ID: 27087367; 28060768; 36252352
Should the use of this material result in one or more scientific
publication(s) the recipient agrees to cite the paper(s)： PubMed ID: 27087367; 28060768; 36252352
Construct Map ：
 
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